rabbit mouse anti-trim28 antibodies (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
rabbit mouse anti-trim28 antibodies
Rabbit Mouse Anti Trim28 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+mouse+anti-trim28+antibodies/mouse+anti-rabbit+IgG-B/pmc05157023-149-52-55
Average 90 stars, based on 1 article reviews
Rabbit Mouse Anti Trim28 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+mouse+anti-trim28+antibodies/mouse+anti-rabbit+IgG-B/pmc05157023-149-52-55
Average 90 stars, based on 1 article reviews
rabbit mouse anti-trim28 antibodies - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation"
Article Title: Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation
Journal: Scientific Reports
doi: 10.1038/srep39049
Figure Legend Snippet: ( A , B ) Co-immunoprecipitation of interacting proteins with REST. Co-immunoprecipitation was performed by mixing V5 antibody-conjugated beads with cell lysates of HEK293FT cells transfected with pcDNA_V5 or pcDNA_REST-V5 vectors. mRNA processing/splicing associated target proteins including ALYREF and HnRNP Q ( A ) and transcription-related target proteins including HDAC5, NPM1, NCL, PARP1, and TRIM28 ( B ) were confirmed by co-immunoprecipitation coupled with western blotting. Arrows indicate the target proteins. Full-length blots are included in the . ( C , D ) Co-localization of REST with interacting proteins such as ALYREF, HnRNP Q ( C ), HDAC5, NPM1, NCL, PARP1, and TRIM28 ( D ). HEK293FT cells transfected with REST-V5 expressing vector were fixed after 24 h, followed by probing with the indicated target specific antibody and V5 antibody. Images analyzed at magnification of 400x. Scale bar indicates 10 μm.
Techniques Used: Immunoprecipitation, Transfection, Western Blot, Expressing, Plasmid Preparation
Figure Legend Snippet: ( A ) The overlap between differentially expressed transcripts following knockdown of REST and REST-interacting proteins is depicted as Venn diagrams. Blue and green circles indicate transcripts regulated by REST and TRIM28, respectively. ( B ) Gene Ontology (GO) analysis using gene sets upregulated by REST and TRIM28 knockdown. ( C ) Network analysis of gene set upregulated by REST and TRIM28 knockdown. Edges were drawn based on the STRING databases. The node color indicates the clustering coefficency. Fisher’s test was used for statistical analysis.
Techniques Used:
Figure Legend Snippet: ( A ) Physical interaction between REST and TRIM28 in the mouse brain lysates. ( B ) SH-SY5Y cells transfected with an indicated siRNA. Levels of REST, TRIM28 and actin were assessed using western blot. Full-length blots are included in the . ( C ) mRNA levels of CTNND2 in SH-SY5Y cells transfected with the indicated siRNA are indicated in the histogram. Three independent experiments were performed, and the t-test was used for statistical analysis. (* P < 0.05; ** P < 0.01; *** P < 0.001).
Techniques Used: Transfection, Western Blot
Figure Legend Snippet: ( A ) Primary neurons were obtained from mouse embryo and maintained in the culture plate. The primary neurons were fixed on days 0, 2 and 4, followed by probing with Phalloidin. Scale bar indicates 50 μm. ( B – D ) mRNA levels of REST ( B ), TRIM28 ( C ) and protein levels of REST, TRIM28 ( D ) were accessed in the primary neurons on days 0, 2 and 4. ( E ) mRNA level of CTNND2 accessed in the primary neurons on days 0, 2 and 4. Three independent experiments were performed to analyze alteration of genes at mRNA level, and the t-test was used for statistical analysis. (* P < 0.05; ** P < 0.01; *** P < 0.001).
Techniques Used:
Figure Legend Snippet: ( A ) Representative images of differentiated CAD cells transfected with pmCherry-N1 and siRNAs. Scale bar indicates 30 μm. ( B ) The longest neurite length normalized to soma diameter in siRNA-mediated knockdown groups was normalized to that in the control group (n = 50 for control siRNA, 35 for REST siRNA, 33 for TRIM28 siRNA, 41 for REST siRNA + TRIM28 siRNA). ( C ) Representative images of cultured primary cortical neurons transfected with pmCherry-N1 and siRNAs. Scale bar indicates 20 μm. ( D ) The longest neurite length normalized to soma diameter in siRNA-mediated knockdown groups was normalized to that in the control group (n = 27 for control siRNA, 22 for REST siRNA, 19 for TRIM28 siRNA). The t-test was used for statistical analysis. (* P < 0.05; ** P < 0.01; *** P < 0.001) ( E ) REST and TRIM28 might repress the expression levels of CTNND2 in neural progenitor cells. As the neural progenitor cells differentiate, levels of REST and TRIM28 decrease, which may induce expression of CTNND2 as a result of the loss of transcriptional suppression by REST and TRIM28.
Techniques Used: Transfection, Cell Culture, Expressing
Related Articles
Immunoprecipitation:Article Title: Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation Article Snippet: Antibodies for immunoprecipitation and western blot were from the companies as follows; rabbit anti-REST antibodies (Millipore, Abcam), mouse anti-V5 antibody (Sigma), Western Blot:Article Title: Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation Article Snippet: Antibodies for immunoprecipitation and western blot were from the companies as follows; rabbit anti-REST antibodies (Millipore, Abcam), mouse anti-V5 antibody (Sigma), Transfection:Article Title: Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation Article Snippet: Antibodies for immunoprecipitation and western blot were from the companies as follows; rabbit anti-REST antibodies (Millipore, Abcam), mouse anti-V5 antibody (Sigma), Expressing:Article Title: Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation Article Snippet: Antibodies for immunoprecipitation and western blot were from the companies as follows; rabbit anti-REST antibodies (Millipore, Abcam), mouse anti-V5 antibody (Sigma), Plasmid Preparation:Article Title: Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation Article Snippet: Antibodies for immunoprecipitation and western blot were from the companies as follows; rabbit anti-REST antibodies (Millipore, Abcam), mouse anti-V5 antibody (Sigma), Cell Culture:Article Title: Interactomic analysis of REST/NRSF and implications of its functional links with the transcription suppressor TRIM28 during neuronal differentiation Article Snippet: Antibodies for immunoprecipitation and western blot were from the companies as follows; rabbit anti-REST antibodies (Millipore, Abcam), mouse anti-V5 antibody (Sigma), |